منابع مشابه
Bayesian Modeling of Biomolecular Assemblies with Cryo-EM Maps
A growing array of experimental techniques allows us to characterize the three-dimensional structure of large biological assemblies at increasingly higher resolution. In addition to X-ray crystallography and nuclear magnetic resonance in solution, new structure determination methods such cryo-electron microscopy (cryo-EM), crosslinking/mass spectrometry and solid-state NMR have emerged. Often i...
متن کاملAutomated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta
Cryo-EM has revealed the structures of many challenging yet exciting macromolecular assemblies at near-atomic resolution (3-4.5Å), providing biological phenomena with molecular descriptions. However, at these resolutions, accurately positioning individual atoms remains challenging and error-prone. Manually refining thousands of amino acids - typical in a macromolecular assembly - is tedious and...
متن کاملCryo-EM enters a new era
Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.
متن کاملCryo-EM structure of a transcribing cypovirus.
Double-stranded RNA viruses in the family Reoviridae are capable of transcribing and capping nascent mRNA within an icosahedral viral capsid that remains intact throughout repeated transcription cycles. However, how the highly coordinated mRNA transcription and capping process is facilitated by viral capsid proteins is still unknown. Cypovirus provides a good model system for studying the mRNA ...
متن کاملCryo-EM of viruses and vaccine design.
Knowledge of the atomic resolution structures of viruses can be a powerful tool for vaccine discovery and design. X-ray crystallography has long served as an invaluable method for virus structure determination at high resolution, but over the past decade, cryoelectron microscopy (cryo-EM) has begun to emerge as a complementary method that can also provide this kind of information. Crystallograp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.11.2571